Title

Safety of Oral Administration of PMF a Fraction Derived From Camel Urine- in Cancer cell

The research interest of Dr. Samy Selim is lies in Microbiology and Natural Products. During my PhD at the Suez Canal University (2002), I studied the mechanisms of antibiotic resistance pathogenic bacteria, through analysis at multiple organizational levels (e.g. molecular biology, plasmids profile, genomic level), I contributed to understanding the molecular mechanisms underlying the antibiotic resistance effect on human health. I have also optimized various protocols, that turned out to be of interest to other researchers, and have resulted in local and international collaborations. All together my research has resulted in 112 publications and my promotion to Professor at Suez Canal University, Egypt. I have received a best researcher award at Jouf University, KSA (2013 and 2015). I have performed research stays at Pecs University-Hungary, Institute of Molecular Biology-Slovakia and University of Debrecen-Hungary. I have gained research funding at Western Washington University-USA, Antwerp University- Belgium and King Abdulaziz University-Saudi Arabia (Consultant Center of Excellence in Prophetic Medicine Applications Research).

Title

Metabolic analysis of various Phoenix dactylifera dates: Nutritional value

Abstract

In this study, we performed bioactivity analyses and metabolic profiling of date fruits of 12 cultivars from Saudi Arabia to assess their nutritional value. Our results showed that the date extracts from different cultivars have different free radical scavenging and anti-lipid peroxidation activities. Moreover, the cultivars showed significant differences in their chemical composition, e.g., the phenolic content (10.4–22.1 mg/100 g DW), amino acids (37–108 μmol•g−1 FW) and minerals (237–969 mg/100 g DW). Principal component analysis (PCA) showed a clear separation of the cultivars into four different groups. The first group consisted of the Sokary, Nabtit Ali cultivars, the second group of Khlas Al Kharj, Khla Al Qassim, Mabroom, Khlas Al Ahsa, the third group of Khals Elshiokh, Nabot Saif, Khodry, and the fourth group consisted of Ajwa Al Madinah, Saffawy, Rashodia, cultivars. Hierarchical cluster analysis (HCA) revealed clustering of date cultivars into two groups. The first cluster consisted of the Sokary, Rashodia and Nabtit Ali cultivars, and the second cluster contained all the other tested cultivars. These results indicate that date fruits have high nutritive value, and different cultivars have different chemical composition.

Title 2

Anticancer, anti-inflammatory, antioxidant and anti-Angiogenic activities of diosgenin isolated from Costus speciosus (Koen.)

Abstract

Costus speciosus is an important medicinal plant widely used in several indigenous medicinal formulations. The present study was conducted to evaluate the in vitro anticancer, anti-inflammatory, antioxidant and anti-angiogenic activities of diosgenin isolated from C. speciosus (Koen.) Sm. The diosgenin isolated from C. speciosus by HPTLC. The results indicated that the treatment of HepG2 cells with the sample resulted in a cytotoxic effect as concluded from the IC50 value 32.62 µg/ml, while the treatment of MCF-7 cells resulted in high inhibition in the cell viability, and resulted in an IC50 value of 11.03µg/ml. The levels of DR4 and caspase-3 were significantly increased (P <0.01) in MCF-7 cells treated with the tested sample compared to untreated cells. Also, the results demonstrated that LPS stimulated TNF-α generation in RAW 264.7 macrophages culture supernatant up to 3.7-fold of the control and that diosgenin treatment (50 µg/mL) resulted in a highly significant inhibitory effect on LPS-stimulated TNF-α (P<0.01) in a similar manner to methotrexate inhibitory effect. The diosgenin possessed an effective antioxidant scavenging affinity against DPPH radicals as compared with the standard antioxidant activity of vitamin C. The results presented here may suggest that diosgenin isolated from C. speciosus possess anticancer, apoptotic and inhibitory effects on cell proliferation.

Dr. Soad K. Al Jaouni is a Professor & Consultant of Hematology and Professor/Consultant of Pediatric Hematology/Oncology, Faculty of Medicine, King Abdulaziz University Hospital a Tertiary care Medical Center, King Abdulaziz University (KAU), Jeddah, Kingdom of Saudi Arabia, with more than 29 years experience in Hematology/Ped.

Oncology with multi-disciplinary comprehensive care to improve the quality of life of patients since 1990.

November 1989 had a certificate of The Royal College of Physician and Surgeon of Canada. Specialty Hematology, Toronto University, Canada. February 1990 and March 2006 had a Fellow Certificate of The Royal College of Physician and Surgeon of Canada in the Division of Medicine.

June 1990 had a Clinical Fellowship in Pediatric Hematology/Oncology Sick Kid Hospital, Toronto University Canada. Maintenance Certificate 2005.

1990 Initiate the Division of Pediatric Hematology/Oncology at King Abdulaziz University Hospital (KAUH), Faculty of Medicine, KAU.

1993 up to present Head Division of Pediatric Hematology/Oncology at KAUH.

1999-2001, 2002, 2007, 2009-2016 Head Section of Hematology Research Lab at King Fahd Medical Research Canter, KAU.

2012 until present: Director & CEO of Scientific Chair of Prophetic Medicine in Faculty of Medicine, KAU

2016 up to present President of Saudi Society of Hematology

2018 Member of Selection Committee for the International Prize of King Faisal in Medicine

Member of 4 Editorial Board of respectable Journals

Awards: Received different Awards in Medicine, Research and Community Services.

Yearly participate and organized International & Local Conferences & workshop with more than 380 research, lately participate in the conferences as keynote speaker and an honorable Organizing Committee Member (OCM) and Advisory Committee. Published more than 160 publications in respectable journals.

Title

Effects of Phoenix dactylifera Ajwa in Evidence-Based Medicine to Improve the Quality of life

Abstract

Date fruits are used as the sole food in many countries over the history.Published data on Phoenix dactylifera palm date Ajwa have beneficial nutritional and medical values.

Controlled study between 2008-2018 showed that regular intake of Ajwa results in a significant improvement in the quality of life in Pediatric Cancer patients at King Abdulaziz University Hospital over 10 years with decreased infection rate, hospital admission for fever neutropenia and improved the treatment out come.

Ajwa have some sort of cardiac protection and treatment. Ajwa intake did not have any effect on tumor growth and have cell protection from cancer treatment. Date palm has novel findings and future direction for food and drug discovery.

DR. MOUSA is a tenured professor of Pharmacology at Albany College of Pharmacy, Executive VP and chair of PRI at Albany, NY. Dr. Mousa is the president of vascular Vision Pharmaceuticals and founders of several spin-off Pharmaceutical and biotechnology companies focusing on the treatment of various blood, vascular disorders, hematological and Oncological diseases. He held a senior scientist and a research fellow at DuPont Pharmaceuticals and DuPont Merck for two decades. He is also a Visiting Scholar at the Johns Hopkins University and holds academic appointments of Adjunct Professor in the State University of New York at Buffalo/Albany, and Rensselaer Polytechnic Institute. Awarded the 2017 Kuwait Foundation for Advancement in Sciences (KFAS) Laureate for Applied Sciences in Medicine. Elected and inducted in 2018, a fellow of the National Academy of Inventors (FNAI).
Dr. Mousa’s work reported in over 1,000 peer-reviewed publications and holds over 400 US & International Patents. Dr. Mousa has contributed to the discovery and development of many products/clinical candidates: Cardiolite (myocardial imaging), Marluma (breast cancer detection), the ultra-short acting beta-adrenergic receptor blocker Esmolol (Breviblock), anti-dyslipidemia (first oral anti-PCSK9) and oral anti-thrombotic Drugs. He established state of the art Pharmaceutical Research Institute (PRI) focusing on translational research. PRI utilizes various forms of enabling technologies ranging from biotechnology, nanotechnology, stem cells, drug design, pharmacokinetics, Pharmacodynamics, and Pharmacogenomics toward personalized or precision medicines. He also advanced several key concepts in vascular, cardiovascular, and hematology/ oncology diseases from the bench to the bedside.

Title 1

Impact of Nanotechnology on the future of pharamaceuticals and Nutraceutical: The Road toward precesion Medicines

Abstract

Over the past decade, evidence from the scientific and medical communities has demonstrated that nanobiotechnology and nanomedicine have tremendous potential to affect numerous aspects of cancer and other disorders in term of early diagnosis and targeted therapy. The utilization of nanotechnology for the development of new Nano-carrier systems has the potential to offer improved targeted delivery through increased solubility and sustained retention and more importantly active targeting. One of the major advantages of this innovative technology is its unique multifunctional characteristics. Targeted delivery of drug incorporated nanoparticles, through conjugation of site-specific cell surface markers, such as tumor-specific antibodies or ligands, which can enhance the efficacy of
the anticancer drug and reduce the side effects. Additionally, multifunctional characteristics of the Nano-carrier system would allow for simultaneous imaging of tumor mass, targeted drug delivery and monitoring (Theranostics).

A summary of recent progress in nanotechnology as it relates to nanoparticles and drug delivery will be reviewed. Nano Nutraceuticals using combination of various natural products provide a great potential in diseases prevention. Additionally, various Nanomedicine approaches for the detection and treatment of various types of organ specific
delivery, vascular targeting, and vaccine will be briefly discussed.

Reference: Mousa SA, Bawa R, and Audette GF (Editors): The Road from Nanomedicine to Precision Medicine, Jenny Stanford Publishing (2020) – 1200+ pages, 36 chapters [ISBN 978-981-4800-59-4 (Hardcover), 978-0-429-29501-0 (eBook)].

Title 2

Protective Roles of Thymoquinone Nanoformulations: Potential Nanonutraceuticals in Human Diseases

Abstract

Nanotechnology has the potential to improve bioavailability and drug delivery for bioactive compounds derived black seed (Thymoquinone, TQ) for the effective management of different human diseases.
Therefore, numerous Nanoformulations have been developed improved oral bioavailability of Thymoquinone. The current study gives a review of the applications of Nanomedicine to enhance the biological activities of TQ to control different diseases in several in vivo studies as preliminary investigation for human diseases treatment by Nano-TQ. Nano-TQ effectively augments the anticancer roles of doxorubicin by upregulation of P53, downregulation of Bcl2 and potentiated paclitaxel’s apoptosis in MCF-7, breast cancer cell. Moreover, Nano-TQ protects against diabetes, inflammation, CNS, and hepatotoxicity mainly by enhancement of organs’ antioxidant status. From the current studies, we can conclude that Nano-TQ is a promising nutraceutical for human health in the prevention and treatment of various disorders.

Title 3

Uncovering Novel Compositions Derived from Camels and Health Benefits

Abstract
We developed a camel-inspired medication to treat sickle cell disease, a sulfated non-anticoagulant low-molecular weight heparin (SNACH). Camel-derived SNACH has been shown pre-clinically to me more efficacious than the currently available therapies for sickle cell disease and sickle cell crisis due in part to its multi-model mechanisms of
action. While current therapies attempt to treat sickle cell disease via one mechanism, SNACH has a number of independent mechanisms of action that serve to rapidly resolve or prevent sickle cell crises by acting as an anti-sickling, anti-thrombotic, anti-selectin and anti-adhesive. Nanoformulated SNACH with camel milk lactoferrin demonstrated high oral bioavailability in Sickle cell, cancer, inflammatory, and infectious diseases. Dr. Mousa has also applied his expertise in the area of Nanoformulation to use natural products, such as mushroom and shellfish-derived polycationic chitosan or camel milk, lactoferrin to Nanoformulate prophetically recommended products such as ajwa dates, musk, black seed, and others for effective development and delivery as pharmaceutical products to treat various diseases.

Dr. Mousa continues these efforts in his laboratories at the Pharmaceutical Research Institute in New York as well as with his collaborators in Saudi Arabia at the Prophetic Medicine Chair and across the world.

Dr. Amir Sheikh
MBBS, BSc, DRCOG, IFM-CP, MRCGP
Dr. Amir Sheikh is currently practicing as a Consultant in Family Medicine. He is also the Regulatory Affairs lead in the British Cupping Society and a Medical Cupping Practitioner with over 12 years of experience. He is part of a UK based team delivering education and training to healthcare professionals. Currently he is collaborating in research in cupping therapy, as well as working to promote its integration in the conventional healthcare setting. He has recently become a certified practitioner in Functional Medicine with the Institute of Functional Medicine (IFM) in the USA. His area of interest is in the delivery of holistic and integrated care with a focus on wellness, primary prevention and treating the root causes of disease and illness with the aim of restoring health. His main area of interest in cupping therapy is in promoting safety and standardisation.

Title

Challenges to the Professionalisation and Standardisation of Cupping Therapy – a UK Perspective

Abstract

Cupping therapy in the United Kingdom has faced various challenges over the last decade. Despite being accepted as an alternative therapy by voluntary regulatory authorities as well as many communities within the UK it still faces scepticism regarding its merits and generalised acceptance. Amongst the obstacles facing standar disation and professionalisation of cupping therapy include variations in practices, differing backgrounds of practitioners delivering the therapy as well as incongruent curricula between training organisations. This has transpired into cases of litigation, namely malpractice and disciplinary matters amongst cupping practitioners.
Conclusion
As the British Cupping Society (BCS) we have endured to tackle the aforementioned issues and challenges. We therefore aim to present an overview of these challenges through discussion of cases and controversies as well as providing an overview of our involvement with public health authorities within the UK. In conclusion we aim to
provide a roadmap of solutions and how the current situation can be addressed through further research and collaboration.

Dr. Ahmed Younis is currently the Director of Learning and Teaching in the Faculty of Health and Social Care Sciences of St. George’s University of London and Kingston University- as well as acting Associate Dean of Education and Consultant of Rehabilitation Management in the Ministry of Health in Palestine. Dr. Younis is one of the UK’s leading figures in complementary medicine, he is also one of the most internationally renowned figures in the revived practice of cupping therapy (Hijamah). Alongside his work as the president of the British Cupping Society he has led and developed workshops on teaching healthcare professionals cupping therapy – both in the UK and abroad. Dr Younis was also sought out to supervise a PhD research on cupping’s efficacy.

Besides holding responsibilities as an operational manager for St. George’s and Kingston universities, he is also the Chair of several Examination Boards for several undergraduate and postgraduate programs. Previously, he led as Deputy Head of the School of Rehabilitation Sciences in the Faculty of Health and Social Care Sciences of St. George’s University of London and Kingston University.

Dr. Younis holds several visiting professorships from Bezmialem Vakif University: Istanbul, Turkey, University of Hargeisa: Somaliland, Goll is University: Somaliland, Amoud University: Somaliland and Biruni
University: Istanbul, Turkey.

Title

Complementary medicine vs Alternative medicine: Evidence-based cupping therapy

Abstract

Introduction and Background:
Complementary Medicine is both the ancient and contemporary approach to patient management and treatment. However, the truth is lost between complementary medicine and conventional medicine- in relation to effectiveness. Complementary medicine, unlike conventional medicine, lacks evidence to support its effectiveness.
Complementary medicine is still used by most of the global population; a notable example is that of cupping therapy which has been used since the Ancient Egyptians circa 1500 BC- until the present day. Cupping therapy is classified as a complementary medicine and the purpose of this presentation is to provide participants with some evidence of the effectiveness of cupping therapy.

Aim:
The aim of this presentation is to provide some evidence-based practice on the effectiveness of cupping therapy – based on patient’s perception and the outcome of pre & post-cupping therapy research.

Methods:
A variety of methods were used in this presentation from survey design to investigate the perception of patients towards cupping therapy to pre and post- design to examine the significant difference in pain level before and after cupping in relation to knee pain and migraine.

Results:
The results show that the majority of patients of this study (46.3%) seek cupping therapy because conventional medicine fails to treat their conditions and as a preventive Measure (72.1%). Patients also stated their preference for cuppings comes from reasons to do with religious and traditional beliefs.
The pain level in patients, after Cupping therapy was performed, was significantly decreased in migraine P= 0.001 and knee pain P=0.001.

Conclusion:
Although there is clear evidence that cupping therapy decreases the
level of pain in some conditions, the path is still too long to solely depend only on cupping therapy – or any other complementary medicine. There is an urgent need for further research to provide substantial evidence in the field of complementary medicine and until this time governments worldwide should provide regulation to the practice of complementary medicine in collaboration with the World Health Organisation.

President of American society of Traditional Science, Chicago, Illinois, USA
Dean of Faculty of Physical Therapy, Suzie Canal University, Egypt Former Dean of Faculty of Physical Therapy, South Valley University, Egypt
Prof. of Physical Therapy, Faculty of Physical Therapy, Cairo University.

Dr. Amir Saleh is currently the professor who is responsible for Alternative therapy research at Faculty of Physical Therapy, Cairo University.

He’s also a supervisor at Cupping Therapy researches in The Family Medicine department at the faculty of Medicine, Suez Canal University.

Besides his responsibilities as a president of American society of traditional science, he’s also the director of Alex Total Wellness centers at Illinois, USA.

Dr. Saleh originally graduated from Cairo University. Following his graduation, he gained his experience in Cairo, Egypt.

He worked for four years in a military hospital then became a lecturer at faculty of Physical therapy, then moved to Saudi Arabia and stayed there for a year, then got his Physical therapy license from Tennessee, USA. He then moved to Chicago and got another license from the state of Illinois.

Dr. Saleh finished his masters, PhD. from Chicago medical school, and then he joined a post-doctoral program for alternative medicine from the same school.

Title

The total theory of Cupping Therapy

Abstract

The new scientific method in cupping therapy. This methodology is based on a homogeneous stability rule (Homeostasis), whereby the body alone is considered to be integrated with all of its organs and systems. Its treatment can only be achieved with balance, complementarity, and effective job communication among all its systems.
This methodology can be summarized on the following basis
1- This radical holistic therapy depends on the anatomical division of the blood and nervous pathways and follows these pathways to purify the internal biological environment from pathogenic and inflammatory causes.
2- Each patient has his own complaint, and there are apparent illness and others within it that affect each other. Dealing with the known illness is through the established principles and therapeutic principles. As for the unknown, it is dealt with through the research points, the patient’s complaint, and his sick history.
3- Chronic diseases are chronic due to the presence of pathogens that led to their chronicity. These factors must be dealt with to remove them or reduce their effects and deal with diseases that can arise from the effects of this time as dependent diseases and not original.
4- The close relationship between mental and physical illness.
5- Near and far places of pain are directly related to the causes of illness and the basis for monitoring the disease.
Knowing the patient’s sick history and knowing all the surrounding conditions that may be related to the occurrence of the disease.

I am a Family Medicine Consultant, a member of the Royal College of General Practitioners, working from London, United Kingdom.

I have a specialist interest in Emergency Medicine, Cardiology, as well as Diabetes. I have completed a Masters in Biomedical Research as well as a Ph.D. in Cardiovascular Disease, specifically in the area of Atrial
Fibrillation. I have published several peer-reviewed articles. Moreover, I have an active interest in Medical Education, as well as acting as a Trainer/Educator for medical students, junior doctors, and registrars.

As a director of the British Cupping Society, whose ultimate aim is to propagate the professionalization and standardization of cupping
therapy both nationally and internationally, we have been instrumental in developing innovative training programs for all types of health care professionals.

We are passionate about introducing Cupping Therapy, a branch of prophetic medicine, into medical schools, and this forms the basis of our presentation at this conference.

Title

Introducing Cupping Therapy into the UK medical curriculum

Abstract

The purpose of this article is to review the appetite for learning cupping therapy and to discuss some of the barriers of introducing Cupping therapy to Medical students. Moreover, we present a summary of cupping knowledge amongst medical students and discuss the teaching methods employed to facilitate learning. We also review our assessment methods and the outcome of these. We also discuss the feedback from students and report in the way it had made the learning experience dynamic and informed.

We demonstrate a thirsty appetite for knowledge regarding cupping therapy, and a strong desire to acquire this. Our pre-course review found knowledge in cupping therapy was poor to very poor (>60% students).
An obstacle to introducing cupping therapy into medical teaching was “the learning was too focussed on cupping therapy”. We overcame this by including other alternative therapies, and presentations from
respective experts.

Our review found blending teaching methods is an effective way to deliver learning, as measured by student feedback, and assessment outcomes. Teaching involved facilitated learning, practical demonstrations,
videos, whilst assessment involved MCQs, reflective essays, posters, audits, and presentations.

We hope that by sharing our experiences from teaching we can use this as a basis for introducing cupping therapy into mainstream medical student learning.

Title

The Effect of Wet Cupping on Blood Hemoglobin Level

Abstract

Although wet cupping is quite a popular procedure that is practiced worldwide, there is no evidence that it may cause anemia. In this observational retrospective pre–post-study, we looked through the previously recorded data of patients who received wet cupping at King Abdulaziz University Hospital. We included who did complete blood count before and immediately after the procedure (n=364). Then, we recorded their blood hemoglobin, red blood cell count, mean corpuscular volume, mean corpuscular hemoglobin and hematocrit before wet cupping, immediately after it, within 3 months after it, then, finally, from 3 months to 6 months after it. Three comparisons took place for each blood test by comparing the pre-reading with each one of the three post-readings. There was no statistically significant difference in any of the pre-post comparisons of those blood tests. However, after keeping only the anemic patients, the comparison showed statistically, but not clinically significant, when comparing the red blood cell count and the hematocrit before and immediately after wet cupping – p-value was 0.000 for both. anemia was not found as a side effect of wet cupping.

Najlaa S. Al-Radadi has completed his PhD from King Abdulaziz University, Saudi Arabia and postdoctoral studies from Taibah University, Saudi Arabia. She is Associate Professor of Inorganic and Green Nano Chemistry in Taibah University. She has published more than 30 papers in reputed journals and conferences. She got a patent for “Novel bioactive complexes of palladium and platinum derived from some macrocyclic ligands and their impact on gentoxcity” in (21/2/2016) from Saudi Patent Office at (KACST) number (4673), She got a second patent for“Green Synthesis of Platinum Nanoparticles Using Saudi s Dates Extract and their Usage on The Cancer Cell Treatment” in (15/6/2017) from Saudi Patent Office at (KACST) number (5422), She Obtained the Innovation and Excellence Award from the World of Knowledge Exchange Authority at the Leadership, Innovation, and Excellence Conference held in Dubai on 13 November 2017 and She Obtained the Excellence and creativity Award in medicine and science from Sayidaty journal, in Saudi Ariba on 27 January 2019.

Title

Environmentally-safe Synthesis of Gold and Silver Nano-Particles with AL-Madinah Barni Fruit and their Applications in the Cancer Cell Treatments

Abstract
Green synthesis of nano-particles has been an exploring research topic in recent years due to their technology use in biological fields. Present work describes an eco-friendly method for the synthesis of silver and gold nano-particles (Ag NPs, Au NPs) of Barni extract was performed by using 2×10-2 M AgNO3 and 1×10-3 M HAuCl4.3H2O with Barni extracts. Barni date are common in Saudi Arabia and most of the cities in Al-Ahsa and Medina. The synthesised nano-particles have been characterised by UV–Vis spectroscopy and the absorption spectroscopy showed a peak at λmax = 450 nm and 543 nm for silver and gold respectively, Transmission Electron Microscopy (TEM), X-Ray Diffraction (XRD), EDX and Fourier Transform Infrared Spectroscopy (FTIR), Thermo Gravimetric Analysis (TGA). The dates mediated synthesis of silver and gold nano-particles are comparatively rapid, eco-friendly, less expensive, and they have several applications like in anti-bacterial therapy in modern medicine. In the vitro anti-cancer activity, evaluation of the newly synthesised silver and gold nano-particles was carried out against human cancer cell lines colon carcinoma cells (HCT-116), hepatocellular carcinoma (HePG-2), breast cells (MCF-7), and human cervical cancer cell (HeLa).

PRESENT APPOINTMENT
Consultant Cardiologist.
Previously, Head of Noninvasive cardiology (for 30 years)
King Fahd Armed Forces Hospital ,Jeddah, K.S.A.

SOCIETIES
* Fellow of the American College of Cardiology
* Member of American Society of Echocardiography
* Member of European Association of Echocardiography
* Fellow of the British Cardiac Society

EDITORIAL BOARD
* Member of the Board of Directors of the Saudi Heart Association.(1995-2005)
* Member of the Editorial Board of several medical journals.

Publications
Author of 60 books on different health issues and 94 papers in peer reviewed medical journals.

Title

The effect of Siwak as evidence based in Medical benefits

Abstract
The maintenance of oral health is of vital interest and can be achieved mainly by mechanical and chemical means. Despite the widespread use of toothbrushes and dentifrices, natural methods of tooth cleaning using herbal “chewing sticks” is common in several parts of the world. Salvadora persica L., also known as Arak (in Arabic) is the most common traditional source of chewing stick (Miswak) and highly recommended by the Prophet Muhammad (PBUH). It is used by Muslim peoples as a natural product for the cleansing of teeth, to ensure the oral and dental hygiene.

Several scientific studies have demonstrated that the miswak (Salvadora persica) possesses antibacterial, anti-fungal, anti-viral, anti-cariogenic, and anti-plaque properties. Other studies have also showed that miswak has anti-oxidant, analgesic, and anti-inflammatory effects. The use of a miswak has an immediate effect on the composition of saliva. Several studies confirmed that the mechanical and chemical cleansing efficacy of miswak chewing sticks are equal and at times greater than that of the toothbrush. The chemical effects of S. persica on dental plaque, sub-gingival microbiota and gingival inflammation have been well demonstrated.

Despite the extensive studies which have probed primarily into the validation of miwak uses in oral care, there is still a dearth of updated compilation and critical analysis of other potential pharmacological properties of S. persica. The evidence-based medical benefits of Siwak will be presented.

Dr. Sohail Akhter is working as Principal scientist at New product and nanomedicines development Global Research & development department, Teva Pharmaceuticals, United Kingdom and Senior experienced visiting fellow at Centre de Biophysique Moléculaire (CBM)-CNRS/ University of Orléans, UPR4301, Orléans, France/ Le Studium Loire Valley Institute for Advanced Studies, Centre-Val de Loire région, France. Since last 4 years Dr. Akhter has also been
serving a Chair memeber of Yousef Abdullatif Jameel Chair of Prophetic Medical Applications (YAJCPMA), Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia. His present research focuses on the optimization of novel synthetic nanomedicines for dendritic cells transfection of mRNA and DNA as therapeutic vaccination and
anti-cancer therapy. Moreover, he is also actively working in the area of combinational anti-cancer nanomedicine in which his specific interest is to investigate the natural bio-actives enhances chemotherapeutics effect of synthetic anti-cancer drugs as nanomedicines. His past research experiences include Senior research fellow, CBM)-CNRS UPR4301Rue Charles Sadron, 45071/Université d’Orléans, Orléans France, Postdoc research fellow at U.S. Food and Drug Administration (US-FDA)/The Centre for Drug Evaluation and Research (CDER)/DPQR, USA and research associate at the Department Pharmaceutics, Utrecht Institute of pharmaceutical sciences, Utrecht University, Netherlands. He has completed his M. Pharm & PhD in Pharmaceutical Sciences (pharmaceutics specialization). Dr. Akhter has authored more than 60 manuscripts in high impact journals. His research interests involve development and characterization of nanomedicines for various therapeutics application, ocular drug delivery, cancer drug targeting, bioavailability enhancement and therapeutic targeting of natural herbal bio-actives, application of biomaterials in drug and gene delivery & targeting, synthetic nanomedicine in cancer vaccination, biopharmaceutics, drug/nanoparticles metabolism, bio-distribution and bio-analysis, liposomes and polymeric nanoparticles and microspheres depot formulation, regulation of complex drug products (including non-biologicals). Dr. Akhter is actively involved in the consultancy of manufacturing, characterization and regulatory guidance required for complex drug products including nanomedicines.

Title

Improved chemotherapeutic efficacy against resistant human breast cancer with co-delivery of Docetaxel and nigella sativa’s bioactive as a cancer-targeted nanomedicine

Abstract

Multi-targeted chemotherapeutics combinations have received considerable interest in recent years for solid tumors chemotherapy. Here, we optimized low molecular weight chitosan (CS) grafted lipid nanocapsules (LNCs) (referred as CLNCs) for co-delivery of Docetaxel (DTX) and Thymoquinone (THQ) for treatment of drug resistant breast cancer. We first screened the size reduction techniques (homogenization vs ultrasonication) then 33-Box-Behnken design was employed to determine optimal conditions of the final LNCs with the desired quality attributes. Uncoated LNCs exhibited a particle size of 141.7±2.8nm (PdI: 0.17±0.02) with %EE of 66.1±3.5% and 85.3±3.1% for DTX and THQ, respectively. The CS functionalization of LNCs improved the uptake and endosomal escape effect and leads to significantly higher cytotoxicity against MCF-7 and triple negative (MDA-MB-231) breast cancer cells. Furthermore, enhanced anti-angiogenic effect was observed with DTX and THQ carrying CLNCs in Chick embryo choriallantoic membrane (CAM) assay.

Nor Hayati Othman obtained her medical degree; MBBS, from University of Malaya in 1981 and Master of Pathology from the University of Malaya in 1987.  She is an anatomic pathologist and had undergone various sub-specialty pathology training; Neuropathology from the University of Western Australia; Dermatolopathology from University of Sydney, Molecular Pathology from the University of Toronto and University of Cape Town. She is a senior consultant pathologist at Hospital Universiti Sains Malaysia and senior professor [VK5] at the Department of Pathology, School of Medical Sciences Universiti Sains Malaysia (USM). She had been head of the department, Deputy Dean, Research Dean, University Senate member and Director for Center of Clinical research at various phases of her carrier. Currently, she is the President for the International Academy of Pathology [Malaysian Division] and a member of Malaysian Medical Council. Her research interests are in cancer prevention especially on gynae cancers. Her relatively recent research interest is on the effect of honey as a cancer preventive agent.

Title

Are There Natural Cancer Vaccines And Have We Missed Them

Abstract

Cancer is on the rise to almost an epidemic state. Among its risk factors include obesity, high sugar diet, diabetes, smoking and numerous chemical carcinogens including xeno-estrogens and environmental pollutants. Prevention is better than cure. There are natural ‘vaccines’ which could help us in preventing from getting cancer. The talk will focus on 5 natural cancer vaccines; among these is 1. reducing risk factors, 2. daily exercise, 3. dietary selectiveness on foods that might prevent cancer including adding 4. doses of honey and 5. curcumin in daily diet. The talk will focus at length on recently concluded extensive research on Honey and its anti-cancer properties. The research showed honey has great potential to be used as a preventive and therapeutic anti-cancer agent. The rate of experimental induced breast cancer progression is reduced in animals in honey treated groups compared to positive control group. The number of tumors developed was reduced and the tumor size was found to be smaller than those not given honey. The histological grading was also better. The mechanism by which honey exerts cancer-preventive and cancer-therapeutic effects is multifold; through the improvement of immunological, hematological, serological response and by activation of the apoptotic mechanism pathway. The pro-apoptotic proteins are up-regulated and the anti-apoptotic proteins are down-regulated.

Associated Professor Medical Bacteriology.Faculty Member, Department of Biology, Taif University, Saudi Arabia

Memberships
* European Association for Microbiology (2012) up to date
* Member of IFUW (2012) up to date
* Saudi Inventors Association
* Saudi Biotechnological Society (2009) up to date
* Saudi Biological Society (2007) up to date
* Saudi Society for Medical Microbiology and Infectious Diseases (2007) up to date
* The New York Academy of Sciences (2006) up to date Awards
* Taif University Prize for the patent 2010
* Taif University Prize for scientific publications 2011

Patents
1- Isolated peptide from camel urine having antimicrobial effect, 2008
2- Antibacterial and Anticorrosion Titanium Nano-Structure Surface for Dental Implant, 2017
3- Therapeutic botanical mouthwash for microbial oral diseases and pain relief, 2017
4- Activated Amoxicillin-gold nanoparticles for treatment of
Multi-drug resistant bacteria, 2018

Conferences
Many international and local symposiums in prophetic medicine,
antibiotic-resistant pathogens, drug delivery, Green nanoparticles in medical applications and alternative medicine.
Research Interests
Green synthesis nanoparticles and its antibacterial activity against MDR and prophetic complementary medicine.

Title

Antibacterial and anticancer activity of camel’s urine peptides. (Patent)

Abstract

Antimicrobial peptides (AMPs) can act as therapeutic agents for infectious disease, and a new treatment strategies for cancer and bacterial infection.In this study extraction and detection of new types of antibacterial peptides from camel urine were done. The Separated peptides have low molecular weights 12.9 and 16.6 kDa, these two peptides, are relatively enzymatic and heat stable. The peptides fractions were separated and identified by electrical migration on acrylamide gel electrophoresis .These urinary-peptides showed antibacterial activity against multi drug resistant bacteria, the kinetics killing rate indicate superior bactericidal activity for two peptides which acts synergistically on both Gram positive and gram negative bacteria within 2-4 h. The antineoplastic activities were determined by tissue culture techniques, the potential of Antimicrobial urinary peptide inhibits 50% of cancer cell in breast, liver, and colon carcinoma with 40.4, 35.2, and 18.1 μg/ml respectively, the colon cell cancer appear very sensitive to camels peptides then others. These unique peptides are thought to be part of the protection mechanisms of camels from bacterial infection of the urinary system and can be used as a human remedy as natural and safe drug to treat bacterial infections and cancers.

Title 2

Biological activity of sider plant (ziziphus spina christi L) against microbial diseases and its essential role of infection and prevention control

Abstract

Antibacterial activity of biosynthesized silver nanoparticles (AgNPs) was significant in therapeutic application of nanotechnology. This research studied an ecofriendly and rapid method for the first time to synthesize silver nanoparticles using Zizyphus spina christi aqueous leaves extract (ZSE), and their antibacterial properties. The extract was found to have the potential to form silver nanoparticles at room temperature within few minutes. The nanoparticles were characterized using different techniques. The UV-visible spectrum showed a peak at 414 nm corresponding to the plasmon absorbance of silver nanoparticles. The transmission electron microscopy (TEM) showed that the formed particles were hexagonal in shape with appreciable Nano size ranging from 21.5 to 59.67 nm. Fourier Transform Infrared Spectroscopy analysis (FTIR) of biosynthesized AgNPs affirmed the role of ZSE as reducing and capping agent of Ag+ ions to AgNPs, and X-Ray Diffraction patterns (XRD) showed that they could be indexed as face-centered-cubic structure of silver. Antibacterial activity of AgNPs was determined by well diffusion and micro plate assay methods, showing inhibition zones of 24 mm, 23 mm, 15 mm and 17 mm against Staphylococcus aureus, Acinetobacter sp., Pseudomonas aeruginosa and Escherichia coli respectively. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) results showed that AgNPs had MIC, MBC of 45, 57 μg/mL, 49, 61 μg/mL, 63, 90 μg/mL and 59, 82 μg/mL against tested pathogen respectively. Furthermore, the green synthesized AgNPs loaded on band-aids exhibited strong antibacterial effect against multi drug-resistant bacteria. These nanoparticles could be used for treating wounds and preparing wound dressing. Such researches are crucial in the demonstration of therapeutic importance of silver nanoparticles as new alternative in medical application.

Doctor of Philosophy (Ph.D.)
Major: Human and Environmental Sciences -¬ Food and Nutrition
Minor: Clinical and Human Nutrition
University of Arkansas Fayetteville, AR 2010
Literature and Research: I have quite a lot of published researches in high impact factor Journals in ISI labeled Journals.

Workshops; such as:
Giving a Workshop on Quality, Health, and Environment Settings for the 3rd Forum of Productive Families, “Getting rid of Toxins in 48 Hours.

Scientific Forums:
The second Jeddah Municipality Health and
Nutrition convention, Scientific Meeting of the department titled
“ lifestyles and proper nutrition”.
Scientific Associations:
General Authority for Food and Drug Administration / Ministry of Health, Membership of the General Authority for Food and Drug
Administration. Society of Friends of patients with diabetes. Women’s Global Relief Organization. Associations in the field of community

Service:
Membership of the new Muslim body
Many world rewards winging
An author of a2 books and another translated one

Title

Pumpkin Seed Oil and Vitamin E Improve Reproductive Function of Male Rats Inflicted by Testicular Injury

Abstract

The principal goal of this study was to investigate the protective role of pumpkin seed oil (PSO) and vitamin E (Vit E) against sodium valproate (SVP)-induced testicular damage in male rats. Forty-five adult male rats were randomly distributed into 5 groups, of 9 animals each. Group (1) was given orally 1ml distilled water/rat and used as negative control and group (2) was orally given SVP (500 mg/kg/day) during the last week of the experiment period to induce testicular damage and used as positive control. The other three protected groups were pretreated by oral administration of PSO at a dose of 40 mg/kg, Vit E at 200 mg/kg and PSO with Vit E respectively for 8 weeks and received SVP during the last week. Blood samples were collected for estimating serum levels of testosterone, Follicle-stimulating hormone (FSH) and Luteinizing hormone (LH). Semen samples were collected for semen analysis. Rats were then sacrificed and male sexual organs were removed and weighed. Tissue lipid peroxidation (LPX) and activity of antioxidant enzymes in testes and histopathology of testes were also performed. The results showed that combination of PSO and Vit E significantly increased serum levels of testosterone, FSH and LH, testes weight and sperm motility, count and vitality and decreased sperm cell abnormalities in rats given SVP. Decreased testicular LPO, increased antioxidant capacity and alleviation of testicular degenerative changes caused by SVP were reported. In conclusion, PSO and Vit E produce protective and antioxidant effects against SVP-induced testicular damage in rats. Therefore, dietary intake of pumpkin seed oil and vitamin E may be useful for male patients who suffer from infertility due to oxidative stress.

Special Infectious Agents Unit, King Fahad Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.

Yousef Abdul Latif Jameel Scientific Chair of Prophetic Medicine
Application, Faculty of Medicine, King Abdulaziz University.

Steve Harakeh biography:
Professor Steve Harakeh received his B.Sc. and M.Sc. from the American University of Beirut (AUB). He was awarded his Ph.D. degree in Microbiology from the University of Surrey, UK. He spent two years as a Postdoctoral Research Fellowship in the Microbiology and Immunology Department, School of Medicine, at Stanford University, USA, and then he was appointed as a Research Associate (Research Assistant Professor) in the same department. Prof. Steve joined the Linus Pauling Institute for Science and Medicine where he worked and published with Professor Pauling who is the only holder of two unshared Noble prizes in the world. He contributed to the teaching many courses in the field of molecular and environmental microbiology and research methodology. Prof. Harakeh is the recipient of several awards and research grants. Published over 140 papers in peer-reviewed journals and contributed to publishing chapters in many international scientific books. He has organized many scientific conferences. He has served on the editorial board and as a reviewer for a wide range of scientific journals.

Title

The Efficacy of Honey as Antibacterial and Ulcer Healing Agent

Abstract

Honey is effective in the treatment of a broad range of wounds including, amongst others, ulcers. It has, also, been known for its antibacterial potential as well as for its antioxidant properties.

Gastric ulcers have been considered as a major problem worldwide. The use of conventional drugs in the treatment of ulcers have had side effects and have not been very effective in many instances. For this reason, new products have been sought. Honey has been used for the treatment of several disorders including gastrointestinal ones especially stomach ulcers. It promotes the healing of ulcers via its antioxidant activity.

Extensive research has been conducted on the antibacterial activity of honey and indicated that honey is potent against both Gram-positive and negative bacteria as well as against Helicobacter pylori which causes ulcers. Honey had a more pronounced inhibitory effect on Gram-negative bacteria (Pseudomonas aeruginosa, Enterobacter spp., Klebsiella) in comparison to commonly used antimicrobial agents. A synergistic effect was achieved upon the application of honey together with the antimicrobial agents in both Gram negative and positive bacteria. Honey resulted in modulation of the bacterial resistance to commonly used antimicrobial agents. In this presentation, I will focus on the antibacterial and ulcer healing activity of honey with emphasis on our published studies.

* Nasra Naeim Ragab Ayuob is currently working as a professor of Medical Histology and Cell Biology at the Faculty of Medicine, Mansoura University, Egypt. She is Egyptian born in September, 1974.

* She was working between (2008 and 2016) as professor of Anatomy/Histology at Faculty of Medicine, King Abdulaziz University, Saudi Arabia. She conducted a scientific cooperation with the Chair /Center of Excellence of Prophetic Medicine Applications from 2016 till present and published many researches in this research area.

* She supervised a scientific project, with a scientific team at the behavioral science unit at King Fahed Medical Research Center (KFMRC), on the effectiveness on aromatherapy in treating the common behavioral health problems at Saudi Arabia between (2015 and 2017).

* Some of her researches were aiming to assess the efficacy of some herbal and traditional substances (orange peel, Usnic Acid, garlic, red grape, raisins, thymoquinon, Musk, Ryhan) in the treatment of some health problem e.g. contact dermatitis, obesity, diabetes mellitus, hypercholesterolemia, depression, anxiety, Alzheimer).

Title

Renoprotective effect of red grape (Vitis vinifera L.) juice and dark raisins against hypercholesterolaemia-induced tubular renal affection in albino rats

Abstract

This study aimed to assess the efficacy of Red grape juice (RGJ) and dark raisins (DR) in protecting the renal tubules against hyper cholesteraemic-induced pathological changes.

Forty albino rats divided into four groups were utilized in this study. They included the control, high cholesterol diet (HCD)-fed, HCD+RGJ-fed, and HCD+DR-fed groups. Blood and insulin levels, lipid profile and kidney functions were assessed at the start of the experiment. The kidney was assessed hisopathologically using light and electron microscopes. The integrity of the renal tubules was assessed immunohistochemically.

The administration of HCD resulted in marked affection of the structure of the renal tubules. Both RGJ and DR, when administered along with the HCD for 12 weeks, improved the lipid profile, kidney functions as well as the histologic and cellular changes-induced by hyper cholesterolaemia in the rats kidney. The effect of raisins was superior to RGJ which might be due to its high contents of fibers and proteins.

This study highlighted the importance of supplementation of red grape and raisins in protection against the harmful effects induced by deposition of fat on the renal tubules’ structure and function.

Title 2

The protective role of musk on salivary glands of mice exposed to chronic unpredictable mild stress.

Abstract

This work aimed to assess the efficacy of musk in relieving the behavioral, biochemical and hippocampal histopathological changes induced by exposure to chronic mild stress in mice and explore the possible mechanism behind this antidepressant-like effect.

Forty male albino mice were divided into four groups: control, a group exposed to chronic unpredictable mild stress (CUMS) and two groups exposed to CUMS and then treated with fluoxetine or musk. Behavioral changes and serum corticosterone levels were assessed at the end of the experiment. Protein and gene expressions of brain-derived neurotropic factor (BDNF) and glucocorticoid receptors (GRs) in the hippocampus were assessed using ELISA and real-time RT-PCR, respectively.

Histopathological examination of the hippocampus and immunohistochemical techniques using glial fibrillary acidic protein (GFAP), Ki67, caspase-3, BDNF and GR were performed.

Results showed that inhalation of musk had an antidepressant-like effect in an animal model of depression. Musk alleviated the behavioral changes and elevated serum corticosterone levels induced by chronic stress. It reduced hippocampal neuronal apoptosis and stimulated neurogenesis in the dentate gyrus.

Musk’s action may be related to upregulation of hippocampal GR and BDNF expressions. Musk is considered a potential antidepressant so it is advisable to assess its efficacy in treating depressed patient.

My name is Ali Hafez El-Far, Associate Professor of Biochemistry, Faculty of Veterinary Medicine, Damanhour University, Egypt. I awarded for several postdoctoral fellowship in in Graduate School of Medical Sciences, Kanazawa Univ., Japan on cancer cell research. Also, in Cell Culture Department, Institute of Experimental Pharmacology & Toxicology, Slovak Academy of Sciences, Slovakia. Postdoctoral researcher in Laboratory of Molecular Bases of Aging, Nencki Institute of Experimental Biology, Polish Academy of Science, Warsaw, Poland in a project entitled ‘’Senolytic Effects of Thymoquinone, Caffeine, and curcumin on Colon (HCT116) and Breast (MCF 7) Senescent Cancer Cells’’. I have practical experience of Western blot, BrdU assay, SA-βgal, cell cycle analysis, cell viability assay, cell migration assay, cell invasion assay, Annexin V assay, MTT assay, UV spectrophotometry, ELISA, and enzyme kinetics.

Title

Senolysis of cancer cells by natural products is one of senescence bright faces: Future plan

Abstract

Cellular senescence, aging, is a process of physiological growth arrest, which can be induced by intrinsic or extrinsic stress signals. Aging is a risk factor for many diseases such as cardiovascular disease, dementia, osteoporosis, osteoarthritis, cancer, and type 2 diabetes. Senescence is characterized cell growth arrest, increased accumulation of senescence-associated-β-galactosidase (SA-β-gal), and upregulation of p53, P-p53, and p21 proteins. Senescence of the cancer cells is considered as a new tool for cancer eradication through inhibition of cell proliferation. Moreover, we suggest using a natural products to induce extensive apoptosis of senescent cancer cells.